antigen human cardiac troponin i ctni  (HyTest)

Journal: Nucleic Acids Research

Article Title: A general strategy to enhance aptamer affinity by suppressing dissociation through symmetric assembly

doi: 10.1093/nar/gkag268

Figure Lengend Snippet: Symmetric trimeric assembly enhances aptamer affinity for VEGF 165 and Troponin I. BLI sensorgrams showing the binding kinetics of ( A ) monomeric H4r3 and ( B ) symmetric homotrimeric shTH4r3 for VEGF 165 , and ( C ) monomeric Tro4 and ( D ) symmetric homotrimeric shTTro4 for troponin I. Concentration-dependent sensorgrams were globally fitted using 1:1 binding model to obtain the kinetic parameters k on , k off , and K d . The experimental curves are shown as solid lines and the fitted curves are shown as black dashed lines. Bar graph comparison of ( E ) k off and ( F ) K d for each aptamer pair. Homotrimeric aptamer assembly dramatically suppressed k off , resulting in >100-fold affinity enhancements for both targets.

Article Snippet: The wild-type SARS-CoV-2 spike protein subunit S1 (mS-protein, Cat. No. 40591-V08B1) and Troponin I (Cat. No. 501-TNNI0010) were purchased from Sino Biological Inc. VEGF 165 protein (his-tagged, Cat. No. VE5-H5248) was obtained from Acro Biosystems.

Techniques: Binding Assay, Concentration Assay, Comparison

Journal: bioRxiv

Article Title: Developmental Hypoxia Increases Susceptibility to Cardiac Ventricular Arrhythmias in Adult Offspring

doi: 10.64898/2026.01.22.701057

Figure Lengend Snippet: Data show the mean ± SD with each sample as dot plots for the protein abundance of phospholamban (PLN, B), phospho-PLN-T17 (C), ratio of phospho-PLN-T17 to PLN (D), cardiac troponin I (cTnI, E), phospho-cTnI (F), ratio of phospho-cTnI to cTnI (G), calsequestrin-1 (H), SERCA2 (I), ratio of SERCA2 to PLN (J), sodium-calcium exchanger 1 (NCX1, K), L-type calcium channel (LTCC, L), ryanodine receptor 2 (RYR2, M), phospho-RYR2-S2808 (N), ratio of phospho-RYR2-S2808 to RYR2 (O) and Connexin43 (Cx43, P). Data was analysed using an unpaired Student’s T-Test for all proteins except PLN, cTnI, NCX and phospho-cTnI/cTnI ratio which used a Mann-Whitney test (data not normally distributed). P<0.05 was considered significant. Normoxia = closed circles, Hypoxia = open circles.

Article Snippet: Membranes were incubated with primary antibodies (PLN [1/5000 dilution] – MA3-922, InVitrogen; phospho-PLN-T17 [1/1000 dilution] – AP0910, ABclonal; cTnI [1/1000 dilution] – MCA1208, Bio-Rad; phospho-cTnI [1/1000 dilution] – MCA2780, Bio-Rad; Calsequestrin-1 [1/5000 dilution] – PA1-913, InVitrogen; SERCA2 [1/2000 dilution] – ab3625, Abcam; NCX1 [1/1000 dilution] – R3F1, Swant; LTCC [1/1000 dilution] – ab2864, Abcam; RYR2 [1/1000 dilution] – ab302716, Abcam; phospho-RYR2-S2808 [1/1000 dilution] – PA5-105712, InVitrogen) overnight at 4°C, before secondary antibody incubation (IRDye® 800CW Goat anti-mouse for PLN, cTnI, phospho-cTnI, NCX1 and LTCC, IRDye® 800CW goat anti-rabbit for phospho-PLN-T17, Calsequestrin-1, SERCA2, RYR2 and phosphor-RYR2-S2808, both 1/20 000 dilution) at room temperature for one hour.

Techniques: Quantitative Proteomics, MANN-WHITNEY

Journal: bioRxiv

Article Title: Developmental Hypoxia Increases Susceptibility to Cardiac Ventricular Arrhythmias in Adult Offspring

doi: 10.64898/2026.01.22.701057

Figure Lengend Snippet: Data show the mean ± SD with each sample as dot plots for the protein abundance of phospholamban (PLN, B), phospho-PLN-T17 (C), ratio of phospho-PLN-T17 to PLN (D), cardiac troponin I (cTnI, E), phospho-cTnI (F), ratio of phospho-cTnI to cTnI (G), calsequestrin-1 (H), SERCA2 (I), ratio of SERCA2 to PLN (J), sodium-calcium exchanger 1 (NCX1, K), L-type calcium channel (LTCC, L), ryanodine receptor 2 (RYR2, M), phospho-RYR2-S2808 (N), ratio of phospho-RYR2-S2808 to RYR2 (O) and Connexin43 (Cx43, P). Data was analysed using an unpaired Student’s T-Test for all proteins except PLN, cTnI, NCX and phospho-cTnI/cTnI ratio which used a Mann-Whitney test (data not normally distributed). P<0.05 was considered significant. Normoxia = closed circles, Hypoxia = open circles.

Article Snippet: Membranes were incubated with primary antibodies (PLN [1/5000 dilution] – MA3-922, InVitrogen; phospho-PLN-T17 [1/1000 dilution] – AP0910, ABclonal; cTnI [1/1000 dilution] – MCA1208, Bio-Rad; phospho-cTnI [1/1000 dilution] – MCA2780, Bio-Rad; Calsequestrin-1 [1/5000 dilution] – PA1-913, InVitrogen; SERCA2 [1/2000 dilution] – ab3625, Abcam; NCX1 [1/1000 dilution] – R3F1, Swant; LTCC [1/1000 dilution] – ab2864, Abcam; RYR2 [1/1000 dilution] – ab302716, Abcam; phospho-RYR2-S2808 [1/1000 dilution] – PA5-105712, InVitrogen) overnight at 4°C, before secondary antibody incubation (IRDye® 800CW Goat anti-mouse for PLN, cTnI, phospho-cTnI, NCX1 and LTCC, IRDye® 800CW goat anti-rabbit for phospho-PLN-T17, Calsequestrin-1, SERCA2, RYR2 and phosphor-RYR2-S2808, both 1/20 000 dilution) at room temperature for one hour.

Techniques: Quantitative Proteomics, MANN-WHITNEY

Journal: Journal of Inflammation Research

Article Title: Establishment of a Novel in vitro Model of Sepsis-Induced Myocardial Injury Using Septic Serum: A Comprehensive Comparative Study

doi: 10.2147/JIR.S523124

Figure Lengend Snippet: Comparison of the degree of injury in various in vitro models of sepsis-induced myocardial injury. ( A – D ) Cell viability of cardiomyocytes treated with LPS, TNF-α and septic serum. ( E ) LDH release level in each group. ( F – H ) The levels of BNP, cTnI and CK-MB in the supernatant of each group. * P < 0.05, ** P < 0.01, *** P < 0.001.

Article Snippet: ELISA kits were also employed to detect markers of myocardial injury in the cell supernatant, including brain natriuretic peptide (BNP) (Elabscience, E-EL-H6166, China), creatine kinase-MB (CK-MB) (CUSABIO, CSB-E05140h, China), and cardiac troponin I (cTnI) (CUSABIO, CSB-E05139h, China).

Techniques: Comparison, In Vitro